An appeals court denied a bid on Thursday by former President Donald Trump and members of his family to avoid testifying as part of New York Attorney General Letitia James's investigation into the Trump Organization's business practices.

The decision, which came from the Appellate Division of the First Judicial Department of Manhattan Supreme Court, marks another legal setback for Trump and his adult children, Donald Trump Jr. and Ivanka Trump, who are ordered to comply with a subpoena issued by James seeking their testimony.

“Once again, the courts have ruled that Donald Trump must comply with our lawful investigation into his financial dealings,” James said in a statement on the appeals court ruling. “We will continue to follow the facts of this case and ensure that no one can evade the law."

TRUMP PAYS $110,000 IN BID TO HAVE CONTEMPT CHARGE PURGED IN NEW YORK

As part of his bid to purge the contempt order in New York, Trump paid a $110,000 fine last week despite not having met all the conditions required to lift a contempt-of-court finding, according to James's office. The order, which was issued on April 25, fines him $10,000 a day until he complies with the court's orders to turn over the documents.

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Trump Jr. operates the Trump Organization with his brother Eric Trump. The former president's daughter was also a former top executive at the family business who departed to take a role in her father's administration.

The Washington Examiner contacted an attorney for the former president.

This is a developing story and will be updated.