Hillary Clinton's campaign released an ad Thursday tying GOP nominee Donald Trump to the Ku Klux Klan and other white supremacist groups.

The ad starts with a quote from the imperial wizard of the Rebel Brigade Knights of the Ku Klux Klan, who said in April, "The reason a lot of Klan members like Donald Trump is because a lot of what he believes is, we believe in. Donald Trump would be best for the job."

Another self-avowed "white nationalist" is quoted in the spot as saying, "Support Donald Trump."

The ad also included a much-discussed quote from former KKK leader David Duke, who said in February, "Running against Donald Trump, at this point, is really treason to your heritage."

Trump declined at first to disavow Duke, and said he had never heard of him. The GOP nominee later backed away and said he disagreed with Duke's beliefs.

"This is who runs Trump's campaign," said the Clinton ad, referring to the Republican candidate's recent hire of former Breitbart News chief Steve Bannon. "If Trump wins, they could be running the country."

The ad comes just hours after Clinton and Trump publicly accused the other of being a bigot.

"Hillary Clinton is a bigot who sees people of color only as votes, not as human beings worthy of a better future," the Republican nominee said Wednesday night at a rally in Jackson, Miss.

Though Trump has previously referred to Clinton's policies as being bigoted against minority voters, this week marks the first time he has come right out and accused the Democratic nominee herself of being a "bigot."

Clinton responded later that evening in an interview on CNN, and said that it is Trump who is the real racist.

"He is taking a hate movement mainstream," she said in reference to the Trump campaign hiring Bannon. "He has brought it into his campaign.

"He is bringing it to our communities and our country, and, you know, someone who has questioned the citizenship of the first African-American president, who has courted white supremacists, who has been sued for housing discrimination against communities of color, who has attacked a judge for his Mexican heritage and promised a mass deportation force is someone who is, you know, very much peddling bigotry and prejudice and paranoia."

The anti-Trump attack ad comes after the pro-Clinton group Correct the Record released a spot attacking the GOP nominee over the same issue.

Clinton is slated to give a speech Thursday afternoon in Reno, Nev., in which she is expected to address Trump's alleged ties to the Klan, the "alt-right" and other white nationalist groups.